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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer were specifically developed to transfect nucleic acids into mammalian cells a...

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Detalhes bibliográficos
Main Authors: Kroeger, Kelly, Collins, Michelle, Ugozzoli, Luis
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2763291/
https://ncbi.nlm.nih.gov/pubmed/19229174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1026
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