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The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

It is becoming increasingly apparent that electroporation is the most effective way to introduce plasmid DNA or siRNA into primary cells. The Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer were specifically developed to transfect nucleic acids into mammalian cells a...

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Autori principali: Kroeger, Kelly, Collins, Michelle, Ugozzoli, Luis
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2763291/
https://ncbi.nlm.nih.gov/pubmed/19229174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1026
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