cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA–protein fusions

We report a robust display technology for the screening of disulfide-rich peptides, based on cDNA–protein fusions, by developing a novel and versatile puromycin-linker DNA. This linker comprises four major portions: a ‘ligation site’ for T4 RNA ligase, a ‘biotin site’ for solid-phase handling, a ‘re...

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Detalhes bibliográficos
Main Authors: Yamaguchi, Junichi, Naimuddin, Mohammed, Biyani, Manish, Sasaki, Toru, Machida, Masayuki, Kubo, Tai, Funatsu, Takashi, Husimi, Yuzuru, Nemoto, Naoto
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2009
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2760808/
https://ncbi.nlm.nih.gov/pubmed/19528071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp514
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