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Sequential Proteolysis and High-Field FT-ICR MS to Determine Disulfide Connectivity and 4-Maleimide TEMPO Spin-Label Location in L126C GM2 Activator Protein
The GM2 activator protein (GM2AP) is an 18 kDa non-enzymatic accessory protein involved in the degradation of neuronal gangliosides. Genetic mutations of GM2AP can disrupt ganglioside catabolism and lead to deadly lysosomal storage disorders. Crystallography of wild-type GM2AP reveals 4 disulfide bo...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2757923/ https://ncbi.nlm.nih.gov/pubmed/19689113 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac9009935 |
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