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MUTATION OF THE ACTIVE SITE CARBOXY-LYSINE (K70) OF OXA-1 β-LACTAMASE RESULTS IN A DEACYLATION-DEFICIENT ENZYME
Class D β-lactamases hydrolyze β-lactam antibiotics by using an active site serine nucleophile to form a covalent acyl-enzyme intermediate, and subsequently employ water to deacylate the β-lactam and release product. Class D β-lactamases are carboxylated on the ε-amino group of an active site lysine...
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Main Authors: | , , , , , |
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格式: | Artigo |
語言: | Inglês |
出版: |
2009
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2756174/ https://ncbi.nlm.nih.gov/pubmed/19485421 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900448u |
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