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Enhancement of DNA, cDNA synthesis and fidelity at high temperatures by a dimeric single-stranded DNA-binding protein

Bacterial single-stranded DNA-binding proteins (SSBs) are required for DNA replication and repair. We have over-expressed and purified the native form and two His-tagged fusions of the SSB from Thermus thermophilus (TthSSB). The three proteins were found as dimers in solution. They bound in vitro to...

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Bibliografske podrobnosti
Main Authors: Perales, Celia, Cava, Felipe, Meijer, Wilfried J. J., Berenguer, José
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2003
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC275559/
https://ncbi.nlm.nih.gov/pubmed/14602905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkg865
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