Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo

BACKGROUND: Cre recombinase is a common reagent used for the in vivo on/off switching of the expression of target genes flanked by loxP sites. In particular, recombinant TAT-Cre fusion constructs purified from bacteria have been used to promote the cell uptake of the enzyme. However, the recovery of...

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Detalhes bibliográficos
Main Authors: Capasso, Paola, Aliprandi, Marisa, Ossolengo, Giuseppe, Edenhofer, Frank, de Marco, Ario
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2755479/
https://ncbi.nlm.nih.gov/pubmed/19747375
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-9-80
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