Fluorescence Correlation Spectroscopy of Phosphatidylinositol-specific Phospholipase C Monitors the Interplay of Substrate and Activator Lipid Binding

Phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes simultaneously interact with the substrate, PI, and with non-substrate lipids such as phosphatidylcholine (PC). For Bacillus thuringiensis PI-PLC these interactions are synergistic with maximal catalytic activity observed at low to moder...

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Autores principales: Pu, Mingming, Roberts, Mary F., Gershenson, Anne
Formato: Artigo
Lenguaje:Inglês
Publicado: 2009
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2753481/
https://ncbi.nlm.nih.gov/pubmed/19548649
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900633p
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