Quantifying GFP Diffusion in Escherichia coli by Using Continuous Photobleaching with Evanescent Illumination

Fluorescence recovery after photobleaching and fluorescence correlation spectroscopy are the primary means for studying translational diffusion in biological systems. Both techniques, however, present numerous obstacles for measuring translational mobility in structures only slightly larger than opt...

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Bibliographic Details
Main Authors: Slade, Kristin M., Steele, Bridgett L., Pielak, Gary J., Thompson, Nancy L.
Format: Artigo
Language:Inglês
Published: 2009
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2752987/
https://ncbi.nlm.nih.gov/pubmed/19296673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jp810642d
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