Development of a 384-Well Colorimetric Assay to Quantify Hydrogen Peroxide Generated by the Redox Cycling of Compounds in the Presence of Reducing Agents

We report here the development and optimization of a simple 384-well colorimetric assay to measure H(2)O(2) generated by the redox cycling of compounds incubated with reducing agents in high-throughput screening (HTS) assay buffers. The phenol red-horseradish peroxidase (HRP) assay readily detected...

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Détails bibliographiques
Auteurs principaux: Johnston, Paul A., Soares, Karina M., Shinde, Sunita N., Foster, Caleb A., Shun, Tong Ying, Takyi, Harold K., Wipf, Peter, Lazo, John S.
Format: Artigo
Langue:Inglês
Publié: Mary Ann Liebert, Inc. 2008
Sujets:
Original Papers
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2752819/
https://ncbi.nlm.nih.gov/pubmed/18699726
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/adt.2008.151
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