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A Continuous Microplate Assay for Sirtuins and Nicotinamide Producing Enzymes
NAD(+)-dependent protein deacetylases (sirtuins) and other enzymes that produce nicotinamide are integral to many cellular processes. Yet current activity measurements involve expensive and time-consuming assays. Here, we present a spectroscopic assay that circumvents many issues of previous methods...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2009
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2752052/ https://ncbi.nlm.nih.gov/pubmed/19615966 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.07.019 |
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