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Slow cooling of protein crystals

Cryoprotectant-free thaumatin crystals have been cooled from 300 to 100 K at a rate of 0.1 K s(−1) – 10(3)–10(4) times slower than in conventional flash cooling – while continuously collecting X-ray diffraction data, so as to follow the evolution of protein lattice and solvent properties during cool...

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Detalhes bibliográficos
Main Authors: Warkentin, Matthew, Thorne, Robert E.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2746722/
https://ncbi.nlm.nih.gov/pubmed/19798409
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0021889809023553
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