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Cell cycle synchronization of E. coli using the stringent response, with fluorescence labeling assays for DNA content and replication
We describe a method for synchronization of the cell cycle in the bacterium E. coli. Treatment of asynchronous cultures with the amino acid analog, DL-serine hydroxamate, induces the stringent response, with concomitant arrest of DNA replication at initiation. Following release of the stringent resp...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2746677/ https://ncbi.nlm.nih.gov/pubmed/19245839 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2009.02.010 |
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