Wird geladen...
Cell cycle synchronization of E. coli using the stringent response, with fluorescence labeling assays for DNA content and replication
We describe a method for synchronization of the cell cycle in the bacterium E. coli. Treatment of asynchronous cultures with the amino acid analog, DL-serine hydroxamate, induces the stringent response, with concomitant arrest of DNA replication at initiation. Following release of the stringent resp...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2009
|
| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2746677/ https://ncbi.nlm.nih.gov/pubmed/19245839 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2009.02.010 |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|