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Single Tube, High Throughput Cloning of Inverted Repeat Constructs for Double-Stranded RNA Expression

BACKGROUND: RNA interference (RNAi) has emerged as a powerful tool for the targeted knockout of genes for functional genomics, system biology studies and drug discovery applications. To meet the requirements for high throughput screening in plants we have developed a new method for the rapid assembl...

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Hlavní autoři: Hauge, Brian, Oggero, Christopher, Nguyen, Nicole, Fu, Changlin, Dong, Fenggao
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2745663/
https://ncbi.nlm.nih.gov/pubmed/19784365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0007205
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