Quantitative analysis of zeptomole microRNAs based on isothermal ramification amplification

To date, ∼700 microRNA (miRNA) molecules have been identified in humans. Accurate and sensitive quantification of miRNA levels will help unveil their biological functions. Here, we extend the isothermal ramification amplification (RAM) approach to a sensitive and specific real-time assay for quantit...

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Main Authors: Yao, Bo, Li, Juan, Huang, Huang, Sun, Changhong, Wang, Zhao, Fan, Yu, Chang, Qing, Li, Shaolu, Xi, Jianzhong
Formato: Artigo
Idioma:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2009
Assuntos:
Method
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2743063/
https://ncbi.nlm.nih.gov/pubmed/19620236
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1555209
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