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Quantitative analysis of zeptomole microRNAs based on isothermal ramification amplification
To date, ∼700 microRNA (miRNA) molecules have been identified in humans. Accurate and sensitive quantification of miRNA levels will help unveil their biological functions. Here, we extend the isothermal ramification amplification (RAM) approach to a sensitive and specific real-time assay for quantit...
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| Main Authors: | , , , , , , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Cold Spring Harbor Laboratory Press
2009
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2743063/ https://ncbi.nlm.nih.gov/pubmed/19620236 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1555209 |
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