Quantitative analysis of zeptomole microRNAs based on isothermal ramification amplification

To date, ∼700 microRNA (miRNA) molecules have been identified in humans. Accurate and sensitive quantification of miRNA levels will help unveil their biological functions. Here, we extend the isothermal ramification amplification (RAM) approach to a sensitive and specific real-time assay for quantit...

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Bibliographic Details
Main Authors: Yao, Bo, Li, Juan, Huang, Huang, Sun, Changhong, Wang, Zhao, Fan, Yu, Chang, Qing, Li, Shaolu, Xi, Jianzhong
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2009
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Method
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2743063/
https://ncbi.nlm.nih.gov/pubmed/19620236
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1555209
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