Microarray analysis of cytoplasmic versus whole cell RNA reveals a considerable number of missed and false positive mRNAs

With no known exceptions, every published microarray study to determine differential mRNA levels in eukaryotes used RNA extracted from whole cells. It is assumed that the use of whole cell RNA in microarray gene expression analysis provides a legitimate profile of steady-state mRNA. Standard labelin...

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Detalhes bibliográficos
Main Authors: Trask, Heidi W., Cowper-Sal-lari, Richard, Sartor, Maureen A., Gui, Jiang, Heath, Catherine V., Renuka, Janhavi, Higgins, Azara-Jane, Andrews, Peter, Korc, Murray, Moore, Jason H., Tomlinson, Craig R.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2009
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2743046/
https://ncbi.nlm.nih.gov/pubmed/19703940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1677409
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