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Analysis of mutational spectra by denaturant capillary electrophoresis

Numbers and kinds of point mutant within DNA from cells, tissues and human population may be discovered for nearly any 75–250bp DNA sequence. High fidelity DNA amplification incorporating a thermally stable DNA “clamp” is followed by separation by denaturing capillary electrophoresis (DCE). DCE allo...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Ekstrøm, Per O., Khrapko, Konstantin, Li-Sucholeiki, Xiao-Cheng, Hunter, Ian W., Thilly, William G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2742298/
https://ncbi.nlm.nih.gov/pubmed/18600220
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.79
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