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Forced Unfolding of Proteins Within Cells

To identify cytoskeletal proteins that change conformation or assembly within stressed cells, in situ labeling of sterically shielded cysteines with fluorophores was analyzed by fluorescence imaging, quantitative mass spectrometry, and sequential two-dye labeling. Within red blood cells, shotgun lab...

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Detalhes bibliográficos
Main Authors: Johnson, Colin P., Tang, Hsin-Yao, Carag, Christine, Speicher, David W., Discher, Dennis E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2741095/
https://ncbi.nlm.nih.gov/pubmed/17673662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1139857
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