Subunit Analysis of Bovine Heart Complex I by Reversed-Phase HPLC, ESI-MS/MS and MALDI-TOF Mass Spectrometry

An effective method was developed for isolation and analysis of bovine heart Complex I subunits. The method utilizes C18 reversed-phase HPLC and a water:acetonitrile gradient containing 0.1% trifluoroacetic acid. Employing this system, 36 of the 45 Complex I subunits elute in 28 distinct chromatogra...

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Detalhes bibliográficos
Main Authors: Lemma-Gray, Patrizia, Valušová, Eva, Carroll, Christopher A., Weintraub, Susan T., Musatov, Andrej, Robinson, Neal C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2738610/
https://ncbi.nlm.nih.gov/pubmed/18721790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.07.029
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