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Mapping the structure and conformational movements of proteins with transition metal ion FRET
Visualizing conformational dynamics in proteins has been difficult, and the atomic-scale motions responsible for the behavior of most allosteric proteins are unknown. Here, we report that FRET between a small fluorescent dye and a nickel ion bound to a di-histidine motif can be used to monitor small...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2009
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2738593/ https://ncbi.nlm.nih.gov/pubmed/19525958 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1341 |
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