SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs

This protocol describes the reconstruction of biological molecules from the electron micrographs of single particles. Computation here is performed using the image-processing software SPIDER and can be managed using a graphical user interface, termed the SPIDER Reconstruction Engine. Two approaches...

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Bibliografiske detaljer
Main Authors: Shaikh, Tanvir R, Gao, Haixiao, Baxter, William T, Asturias, Francisco J, Boisset, Nicolas, Leith, Ardean, Frank, Joachim
Format: Artigo
Sprog:Inglês
Udgivet: 2008
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737740/
https://ncbi.nlm.nih.gov/pubmed/19180078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.156
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