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Identification of Nucleases and Phosphatases by Direct Biochemical Screen of the Saccharomyces cerevisiae Proteome

The availability of yeast strain collections expressing individually tagged proteins to facilitate one-step purification provides a powerful approach to identify proteins with particular biochemical activities. To identify novel exo- and endo-nucleases that might function in DNA repair, we undertook...

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Bibliographic Details
Main Authors: Ho, Chu Kwen, Lam, Alicia F., Symington, Lorraine S.
Format: Artigo
Language:Inglês
Published: Public Library of Science 2009
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737285/
https://ncbi.nlm.nih.gov/pubmed/19753119
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0006993
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