Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells

Aim. To construct a recombinant eukaryotic expression plasmid containing human calcitonin (hCT) gene and express the gene in murine fibroblast NIH3T3 cells. Materials and Methods. A murine Igκ-chain leader sequence and hCT gene were synthesized and cloned into pCDNA3.0 to form the pCDNA3.0-Igκ-hCT e...

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Main Authors: Li, Xiaolin, Jiang, Guozhong, Wu, Dan, Wang, Xiuli, Zeng, Bingfang
Formáid: Artigo
Teanga:Inglês
Foilsithe: Hindawi Publishing Corporation 2009
Ábhair:
Research Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2729102/
https://ncbi.nlm.nih.gov/pubmed/19696904
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2009/241390
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