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Preparation of Group I Introns for Biochemical Studies and Crystallization Assays by Native Affinity Purification

The study of functional RNAs of various sizes and structures requires efficient methods for their synthesis and purification. Here, 23 group I intron variants ranging in length from 246 to 341 nucleotides—some containing exons—were subjected to a native purification technique previously applied only...

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Detalhes bibliográficos
Main Authors: Vicens, Quentin, Gooding, Anne R., Duarte, Luis F., Batey, Robert T.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2729099/
https://ncbi.nlm.nih.gov/pubmed/19710925
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0006740
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