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Enzymatic measurement of phosphatidic acid in cultured cells
In this work, we developed a novel enzymatic method for measuring phosphatidic acid (PA) in cultured cells. The enzymatic reaction sequence of the method involves hydrolysis of PA to produce glycerol-3-phosphate (G3P), which is then oxidized by G3P oxidase to generate hydrogen peroxide. In the prese...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Biochemistry and Molecular Biology
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2724771/ https://ncbi.nlm.nih.gov/pubmed/19369695 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D900014-JLR200 |
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