MS-qFRET: A quantum dot-based method for analysis of DNA methylation

DNA methylation contributes to carcinogenesis by silencing key tumor suppressor genes. Here we report an ultrasensitive and reliable nanotechnology assay, MS-qFRET, for detection and quantification of DNA methylation. Bisulfite-modified DNA is subjected to PCR amplification with primers that would d...

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Detalhes bibliográficos
Main Authors: Bailey, Vasudev J., Easwaran, Hariharan, Zhang, Yi, Griffiths, Elizabeth, Belinsky, Steven A., Herman, James G., Baylin, Stephen B., Carraway, Hetty E., Wang, Tza-Huei
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2009
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2720185/
https://ncbi.nlm.nih.gov/pubmed/19443857
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.088831.108
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