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Method for the isolation of highly purified Salmonella flagellins.
Ten different Salmonella serotypes were grown in a chemically defined medium supplemented with 0.01% yeast extract. After sedimentation of the cells by centrifugation, flagella were detached by exposure to pH 2 for 30 min at room temperature. The flagellaless cells were removed by centrifugation, an...
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| Publicado no: | J Clin Microbiol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC271874/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4066915/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.22.6.1040-1044.1985 |
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