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Method for the isolation of highly purified Salmonella flagellins.

Ten different Salmonella serotypes were grown in a chemically defined medium supplemented with 0.01% yeast extract. After sedimentation of the cells by centrifugation, flagella were detached by exposure to pH 2 for 30 min at room temperature. The flagellaless cells were removed by centrifugation, an...

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Bibliografiske detaljer
Udgivet i:J Clin Microbiol
Main Authors: Ibrahim, G F, Fleet, G H, Lyons, M J, Walker, R A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1985
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Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC271874/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4066915/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.22.6.1040-1044.1985
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