BRET3: a red-shifted bioluminescence resonance energy transfer (BRET)-based integrated platform for imaging protein-protein interactions from single live cells and living animals

Taking advantage of the bioluminescence resonance energy transfer (BRET) phenomenon, we report the development of a highly photon-efficient, self-illuminating fusion protein combining a mutant red fluorescent protein (mOrange) and a mutant Renilla reniformis luciferase (RLuc8). This new BRET fusion...

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Hlavní autoři: De, Abhijit, Ray, Pritha, Loening, Andreas Markus, Gambhir, Sanjiv Sam
Médium: Artigo
Jazyk:Inglês
Vydáno: The Federation of American Societies for Experimental Biology 2009
Témata:
Research Communications
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2717762/
https://ncbi.nlm.nih.gov/pubmed/19351700
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1096/fj.08-118919
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