Using the Bias from Flow to Elucidate Single DNA Repair Protein Sliding and Interactions with DNA

We perform single-molecule spatial tracking measurements of a DNA repair protein, the C-terminal domain of Ada (C-Ada) from Escherichia coli, moving on DNA extended by flow. The trajectories of single proteins labeled with a fluorophore are constructed. We analyze single-protein dwell times on DNA f...

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Detalhes bibliográficos
Main Authors: Lin, Yihan, Zhao, Tong, Jian, Xing, Farooqui, Zishaan, Qu, Xiaohui, He, Chuan, Dinner, Aaron R., Scherer, Norbert F.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2009
Assuntos:
Protein
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2717288/
https://ncbi.nlm.nih.gov/pubmed/19254550
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.11.021
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