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Chemically modified primers for improved multiplex PCR

Multiplexed PCR, the amplification of multiple targets in a single reaction, presents a new set of challenges that further complicate more traditional PCR set-ups. These complications include a greater probability for non-specific amplicon formation and for imbalanced amplification of different targ...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Shum, Jonathan, Paul, Natasha
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2716723/
https://ncbi.nlm.nih.gov/pubmed/19258004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.02.033
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