Light-Induced Dark States of Organic Fluochromes Enable 30 nm Resolution Imaging in Standard Media

We show that high quantum efficiency fluorophores can exhibit reversible photobleaching. This observation provides the basis for an imaging technique we call reversible photobleaching microscopy. We demonstrate applicability of this technique using antibody labeled biological samples in standard aqu...

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Autors principals: Baddeley, David, Jayasinghe, Isuru D., Cremer, Christoph, Cannell, Mark B., Soeller, Christian
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2009
Matèries:
Biophysical Letter
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2716455/
https://ncbi.nlm.nih.gov/pubmed/19167284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.11.002
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