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Light-Induced Dark States of Organic Fluochromes Enable 30 nm Resolution Imaging in Standard Media
We show that high quantum efficiency fluorophores can exhibit reversible photobleaching. This observation provides the basis for an imaging technique we call reversible photobleaching microscopy. We demonstrate applicability of this technique using antibody labeled biological samples in standard aqu...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2716455/ https://ncbi.nlm.nih.gov/pubmed/19167284 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.11.002 |
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