Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA

We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluate...

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Bibliographic Details
Main Authors: Piqueur, Marian AC, Verstrepen, Walter A, Bruynseels, Peggy, Mertens, An H
Format: Artigo
Language:Inglês
Published: BioMed Central 2009
Subjects:
Short Report
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2716329/
https://ncbi.nlm.nih.gov/pubmed/19583870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-6-95
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