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Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluate...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2716329/ https://ncbi.nlm.nih.gov/pubmed/19583870 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-6-95 |
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