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Rapid and Reliable Detection of Antimicrobial Peptide Penetration into Gram-Negative Bacteria Based on Fluorescence Quenching

In this paper, we describe a rapid flow cytometry method to identify antimicrobial peptides that are internalized into bacterial cells and differentiate them from those that are membrane active. The method was applied to fluorescently labeled Bac7(1-35) and polymyxin B, whose mechanisms of action ar...

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Detalhes bibliográficos
Main Authors: Benincasa, Monica, Pacor, Sabrina, Gennaro, Renato, Scocchi, Marco
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2715650/
https://ncbi.nlm.nih.gov/pubmed/19470515
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.01620-08
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