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Rapid and Reliable Detection of Antimicrobial Peptide Penetration into Gram-Negative Bacteria Based on Fluorescence Quenching

In this paper, we describe a rapid flow cytometry method to identify antimicrobial peptides that are internalized into bacterial cells and differentiate them from those that are membrane active. The method was applied to fluorescently labeled Bac7(1-35) and polymyxin B, whose mechanisms of action ar...

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Autors principals: Benincasa, Monica, Pacor, Sabrina, Gennaro, Renato, Scocchi, Marco
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2715650/
https://ncbi.nlm.nih.gov/pubmed/19470515
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.01620-08
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