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Extended-Spectrum Properties of CMY-30, a Val211Gly Mutant of CMY-2 Cephalosporinase

CMY-30, a Val211Gly mutant of CMY-2 cephalosporinase, was derived by mutagenesis. The hydrolytic efficiency of CMY-30 against expanded-spectrum cephalosporins was higher than that of CMY-2 due to increased k(cat) values. Findings indicate a role of the Ω loop residue 211 in determining the substrate...

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Bibliografiske detaljer
Main Authors: Kotsakis, Stathis D., Papagiannitsis, Costas C., Tzelepi, Eva, Tzouvelekis, Leonidas S., Miriagou, Vivi
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2715598/
https://ncbi.nlm.nih.gov/pubmed/19470510
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00219-09
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