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A homogeneous cell-based bicistronic fluorescence assay for high-throughput identification of drugs that perturb viral gene recoding and read-through of nonsense stop codons

Recoding mechanisms are programmed protein synthesis events used commonly by viruses but only very rarely in cells for cellular gene expression. For example, HIV-1 has an absolute reliance on frameshifting to produce the correct ratio of key proteins critical for infectivity. To exploit such recodin...

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Detalhes bibliográficos
Main Authors: Cardno, Tony S., Poole, Elizabeth S., Mathew, Suneeth F., Graves, Ryan, Tate, Warren P.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2714747/
https://ncbi.nlm.nih.gov/pubmed/19535460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1586709
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