Fluorophore Binding Aptamers as a Tool for RNA Visualization

Fluorescence correlation spectroscopy (FCS) is suitable for the detection of fluorescent molecules in living cells. For the visualization of mRNA, we genetically fused a fluorophore-specific RNA aptamer to the coding mRNA of the green fluorescent protein, as well as to noncoding sequences. Using the...

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Bibliographic Details
Main Authors: Eydeler, Katja, Magbanua, Eileen, Werner, Arne, Ziegelmüller, Patrick, Hahn, Ulrich
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2009
Subjects:
Nucleic Acid
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2711399/
https://ncbi.nlm.nih.gov/pubmed/19413975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.01.041
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