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Kinetic flux profiling for quantitation of cellular metabolic fluxes
This protocol enables quantitation of metabolic fluxes in cultured cells. Measurements are based on the kinetics of cellular incorporation of stable isotope from nutrient into downstream metabolites. At multiple time points, after cells are rapidly switched from unlabeled to isotope-labeled nutrient...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2710581/ https://ncbi.nlm.nih.gov/pubmed/18714301 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.131 |
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