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In Vivo Labeling Method Using a Genetic Construct for Nanoscale Resolution Microscopy
We demonstrate beam scanning-stimulated emission depletion microscopy with in vivo labeled cells. A red emitting fluorescent dye is introduced into membrane protein fused to a multifunctional reporter protein (HaloTag, Promega, Madison, WI) in live cells. This approach allows superresolution stimula...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
The Biophysical Society
2009
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2710050/ https://ncbi.nlm.nih.gov/pubmed/19134467 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2008.09.032 |
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