Identification and Quantitation of Newly Synthesized Proteins in Escherichia coli by Enrichment of Azidohomoalanine-labeled Peptides with Diagonal Chromatography

A method is presented to identify and quantify several hundreds of newly synthesized proteins in Escherichia coli upon pulse labeling cells with the methionine analogue azidohomoalanine (azhal). For the first 30 min after inoculation, a methionine-auxotrophic strain grows equally well on azhal as on...

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Hlavní autoři: Kramer, Gertjan, Sprenger, Richard R., Back, JaapWillem, Dekker, Henk L., Nessen, Merel A., van Maarseveen, Jan H., de Koning, Leo J., Hellingwerf, Klaas J., de Jong, Luitzen, de Koster, Chris G.
Médium: Artigo
Jazyk:Inglês
Vydáno: The American Society for Biochemistry and Molecular Biology 2009
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Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2709250/
https://ncbi.nlm.nih.gov/pubmed/19321432
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M800392-MCP200
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