Identification and Quantitation of Newly Synthesized Proteins in Escherichia coli by Enrichment of Azidohomoalanine-labeled Peptides with Diagonal Chromatography

A method is presented to identify and quantify several hundreds of newly synthesized proteins in Escherichia coli upon pulse labeling cells with the methionine analogue azidohomoalanine (azhal). For the first 30 min after inoculation, a methionine-auxotrophic strain grows equally well on azhal as on...

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Detalhes bibliográficos
Main Authors: Kramer, Gertjan, Sprenger, Richard R., Back, JaapWillem, Dekker, Henk L., Nessen, Merel A., van Maarseveen, Jan H., de Koning, Leo J., Hellingwerf, Klaas J., de Jong, Luitzen, de Koster, Chris G.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2009
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2709250/
https://ncbi.nlm.nih.gov/pubmed/19321432
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M800392-MCP200
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