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Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent protein
mPlum is a far-red fluorescent protein with emission maximum at ∼650 nm and was derived by directed evolution from DsRed. Two residues near the chromophore, Glu16 and Ile65, were previously revealed to be indispensable for the far-red emission. Ultrafast time-resolved fluorescence emission studies r...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Wiley Subscription Services, Inc., A Wiley Company
2009
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2708057/ https://ncbi.nlm.nih.gov/pubmed/19165727 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.66 |
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