A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein

Far-red fluorescent proteins (FPs) are desirable for in vivo imaging because less light is scattered, absorbed, or reemitted by endogenous biomolecules. A new class of FP was developed from an allophycocyanin α-subunit (APCα). Native APC requires a lyase to incorporate phycocyanobilin. The evolved F...

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Udgivet i:Nat Methods
Main Authors: Rodriguez, Erik A., Tran, Geraldine N., Gross, Larry A., Crisp, Jessica L., Shu, Xiaokun, Lin, John Y., Tsien, Roger Y.
Format: Artigo
Sprog:Inglês
Udgivet: 2016
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5007177/
https://ncbi.nlm.nih.gov/pubmed/27479328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3935
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