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Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent protein

mPlum is a far-red fluorescent protein with emission maximum at ∼650 nm and was derived by directed evolution from DsRed. Two residues near the chromophore, Glu16 and Ile65, were previously revealed to be indispensable for the far-red emission. Ultrafast time-resolved fluorescence emission studies r...

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Detalhes bibliográficos
Main Authors: Shu, Xiaokun, Wang, Lei, Colip, Leslie, Kallio, Karen, Remington, S James
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2708057/
https://ncbi.nlm.nih.gov/pubmed/19165727
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.66
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