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Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system.

A test based on the polymerase chain reaction (PCR) was developed for the detection of the Mycobacterium tuberculosis complex in clinical samples. In this test, a 245-bp sequence of the insertion element IS986 was amplified and detected by agarose gel electrophoresis in the presence of ethidium brom...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Clin Microbiol
Hauptverfasser: Kolk, A H, Schuitema, A R, Kuijper, S, van Leeuwen, J, Hermans, P W, van Embden, J D, Hartskeerl, R A
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1992
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270480/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1400955/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.30.10.2567-2575.1992
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