Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system.

A test based on the polymerase chain reaction (PCR) was developed for the detection of the Mycobacterium tuberculosis complex in clinical samples. In this test, a 245-bp sequence of the insertion element IS986 was amplified and detected by agarose gel electrophoresis in the presence of ethidium brom...

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Bibliographic Details
Main Authors: Kolk, A H, Schuitema, A R, Kuijper, S, van Leeuwen, J, Hermans, P W, van Embden, J D, Hartskeerl, R A
Format: Artigo
Language:Inglês
Published: 1992
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC270480/
https://ncbi.nlm.nih.gov/pubmed/1400955
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