Comparison of Tetraploid Blastocyst Microinjection of Outbred Crl:CD1(ICR), Hybrid B6D2F1/Tac, and Inbred C57BL/6NTac Embryos for Generation of Mice Derived from Embryonic Stem Cells

Embryo electrofusion and tetraploid blastocyst microinjection is a modification of the traditional embryonic stem cell (ES cell)-based method to generate targeted mutant mice. Viability of tetraploid embryos is reportedly lower than with diploid embryos, with considerable interstrain variation. Here...

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Autors principals: Kirchain, Sharron M, Hayward, Alison M, Mkandawire, John M, Qi, Peimin, Burds, Aurora A
Format: Artigo
Idioma:Inglês
Publicat: American Association for Laboratory Animal Science 2008
Matèries:
Mouse Models
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2703175/
https://ncbi.nlm.nih.gov/pubmed/18524172
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