Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment.

A polymerase chain reaction with nested primer pairs based on the DNA sequence of the 39-kDa bmp gene of Treponema pallidum subsp. pallidum is described. The method allowed the detection of purified T. pallidum DNA equivalent to the amount of DNA in a single bacterium and was specific for T. pallidu...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Noordhoek, G T, Wolters, E C, de Jonge, M E, van Embden, J D
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1991
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC270245/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1774324/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.29.9.1976-1984.1991
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